Membranes - Glyphosate

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Microtubules helped you at conception - and every day ever since!

Mitosis is the division and replication of the nuclear DNA before cell division occurs. If our microtubules are dysfunctional - then nothing else is going to work well either, certainly not growing a new baby, which is my specialty - prenatal, lactation, and early childhood nutrition counselor and educator.

• The rest of the construction crew: "Microtubules are involved in mechanical support, cytoplasmic organization, and several cellular processes by interacting with diverse microtubule-associated proteins such as plus-end tracking proteins, motor proteins, and tubulin-folding cofactors. A number of the cytoskeleton-associated proteins (CAPs) contain the CAP-glycine-rich (CAP-Gly) domain, which is evolutionarily conserved and generally considered to bind to α-tubulin to regulate the function of microtubules."

If a major regulatory protein needed for cell division is dysfunctional, then new cells are not going to be able to be grown - for wound healing, immune support, or conception of a new life (a baby).

Image:  "Meiosis. Computer artwork of the first meiotic division." (https://www.superstock.com/stock-photos-images/4128R-25424) The yellow curved lines are microtubules directing the movement of the dividing genes within a cell's nucleus. 

Glyphosate residue in health is a topic that has had minimal research. 

Not looking for answers - means that answers that industry or you may not want to hear, or be heard, are not found. Well-funded independent research is needed - 'trusted peer-reviewed journals' are compromised by ghost-written industry funded articles.

Self pay, self order lab testing is available to measure urinary levels of glyphosate residue. Urinary levels of glyphosate has been associated with more severe Non-Alcoholic Fatty Liver Disease.

• Urinary levels of glyphosate residue can be checked, and increased levels were associated with worse Non-Alcoholic Fatty Liver Disease. (Gly.19*, 20*).

Consider testing the urine output for presences of glyphosate residue whether ill, or planning to conceive a baby. Test kits can be ordered by clinicians or through a health company, example (greatplains.mymedlab.com/great-plains-tests/glyphosate–2);

 Reducintg intake of glyphosate residue is not easy as it is is in most commercial foods as many different ingredients. Animals raised with glyphosate crops as feed will likely have glyphosate residue in the meat or dairy products - if it is taken up in protein structure. Lab tests using digestive enzymes and a before and after test for glyphosate residue could quickly show whether there is an increase in free glyphosate, after it may have been released from the digested protein chains.

Wheat, corn, oats, sugar, and soy are all crops grown with glyphosate as herbicides or before harvest to increase crop yield.

Nutrients that may be depleted by glyphosate include minerals such as iron, manganese and magnesium, CoQ10, and vitamin D. Taking extra methyl B vitamin B12, folate and choline might be helpful and di-methylglycine.

Possible aids if excessive glyphosate is a concern: 

• CoQ10 - 200 mg
• Curcumin, a Vitamin D Receptor agonist/activator. 
• Garlic (source of sulfur containing phytonutrients), 
• Magnesium Sulfate, Epsom salt baths, - topical soaks provide a well absorbed form of magnesium & sulfate.
• Vitamin C, 
• Probiotics (fermented foods, yogurt, live active culture pickles), 
• Methyl tetrahydrofolate – (this is the bioactive form of folic acid), 
• Cobalamin – hydroxy or methyl B12, 
• Liposomal Glutathione (GSH) (an antioxidant that we make for ourselves when healthy)
• or NAC, N-acetyl-cysteine is a precursor of glutathione. Whey protein is a very good source, or other protein foods. It is not scarce in the diet. Aged Garlic Extract can also provide S-allylcysteine. Liposomal glutathione can be better absorbed as glutathione is typically broken down in the intestines during digestion. Liposomal forms encase a nutrient in fats, like a membrane and is absorbed as a fat.
• Alpha lipoic acid and Superoxide dismutases (SODs) are additional antioxidant support for the body. (26)), 
• Taurine (another amino acid),
• Manganese containing foods, Pure Maple Syrup contains almost a day's supply in 1/4 cup. 
• Eat organically grown foods when possible. (9)

See: Resistant Starch/Butyrate and Microbiome for more about probiotics and helping our microbiome be healthier species = so they help us defend against pathogenic species and make butyrate for us. Butyrate and niacin activate the GP10 receptor / Niacin Receptor, which helps reduce inflammation by increasing mitochondrial activity and release of excess energy as heat. It also helps increase endolysosomal removal of cellular debris - like a cellular garbage service that also recycles useful nutrients.

Methylation gene differences may leave some people more at risk for epigenetic conditions - changs in the methyl groups attached to DNA, can make them be in an active or an inactive mode. Postural Orthostatic Tachycardia Syndrome, POTS, may be due to epigenetic changes, and therefore may also be reversible! 

Excess formaldehyde from environmental or internal stress chemical production, may be a risk factor. See: Formaldehyde: Health Risks and Dietary and Environmental Sources.

Lack of methyl donor B vitamins may also be a factor, either from deficiency in the diet, increased need due to inflammation, or long term deficiency due to genetic differences in some stage of the methylation cycle. 

Glyphosate may be involved in epigenetic risks.

Epigenetic changes may also be involved in Covid19 or LongCovid - POTS is a focus of the post, and actin is described, another type of regulatory protein. 

• "Glyphosate decreased expression of P16 and TP53 [protective] as well as an increase in the expression of BCl2, CCND1 and P21. Summing up, our results have shown a potential disturbance in methylation processes and gene expression in human PBMCs exposed to glyphosate," (Wozniak et al, 2020)

Glyphosate may be a factor in epigenetic problems - the good news though, is that epigenetic changes can change back to normal - with adequate methyl donor nutrients. 

Methylation gene differences may be a factor in who is more at risk for POTS or other epigenetic related issues. Adequate methyl groups to attach to a DNA strand can put it in an 'off' mode so whatever protein it transcribes would not be made. If it is for inflammatory protein, we might want that to be in the off position during health.

Methyl B12 and folate would be needed instead of standard non-methylated supplements. Choline and betaine are also methyl donors and avoid BPS. It is a problem chemical from environmental and food sources that takes methyl groups. 

When our body has a good detox function misfolded proteins are identified and removed, or cells with misfolded proteins are removed by apoptosis. Defective or infected cells are killed and engulfed by a white blood cell for safe removal. 

Damaged cells from trauma that release intracellular fluid and its contents into the exterior can lead to worse inflammation as it sends out 'damage' signals calling for more white blood cells to come and help clean up or fight an infection. This is a necessary part of health - remove aged cells and make room for new ones without  toxins being released or a malfunctioning cell remaining alive and using nutrients and possibly causing harm in some other way.

• "Ubiquitination is an essential player in maintaining the balance of proteins in eukaryotic organisms, serving to rapidly remove misfolded proteins.[5][6]" (Roberts, et al, 2021)

Disclaimer: This information is provided for educational purposes within the guidelines of fair use. While I am a Registered Dietitian this information is not intended to provide individual health guidance. Please see a [*functional] health professional for individual health care purposes.  

The image shows actin around a fibroblast cell, made visible with a fluorescent dye. The membrane is more like a water balloon and the flexible rod like proteins provide support for the cell which helps it maintain form. The actin may provide directional guidance for other activity including immune functions. 

• "A fibroblast is the most common type of cell found in connective tissue. Fibroblasts secrete collagen proteins that are used to maintain a structural framework for many tissues. They also play an important role in healing wounds. " (https://www.genome.gov/genetics-glossary/Fibroblast)

Collagen is a structural protein that contains a large percent of glycine molecules. It is necessary in tissue of joints and ligaments for cushioning, connecting, and providing structure to our skeleton. 

Glyphosate within a protein chain may cause misfolding and that may lead to misfolding of other proteins leading to tangles instead of the typical supportive organized framework or guiding crane like functions of actin. Kinesin motors are specialized proteins that can move up and down the exterior of some tubules. Other tubules may be wide enough to allow transport within the center of the spiraling group of smaller protein subunits. Visualize Lego building blocks that form a circular spiral staircase instead of straight wall.

Image: Actin made visible with fluorescent dye.  Fig. 5: Swiss 3T3 fibroblasts stained with ActiStain 488 (green), Dapi (blue) and Anti Vinuclin (orange).  Image: A. El Baya, PhD, (https://www.tebu-bio.com/blog/5-ways-to-stain-actin-and-tubulin-from-wb-to-live-cell-imaging/)

More images: https://www.tebu-bio.com/blog/sir-stain-users-experiences-to-stain-actin-tubulin/  

Spike Protein seems to disrupt microtubules in the extracellular fluid, by depolymerization possibly,

Twitter Thread theory by Claudia-Gertrud Krings, @cgkrings, [account suspended, noted 1/12/2022] (45), or due to glyphosate residue. The microtubule problem is not seen in everyone, suggesting to me that there may be a difference in the people - which may be glyphosate incorporated in microtubules instead of glycine. 

Glyphosate and glycine are both similarly shaped amino acids which can be used to make proteins, however the synthetic glyphosate has a side group that disrupts function of a receptor opening/protein fold. Molecules of glycine are small and are often found at the point where a protein chain folds to make a receptor opening - add a glyphosate instead and the receptor opening is permanently blocked - dysfunctional.

Why is this bad? Because the end of microtubules typically contain a glycine based area that helps control pathogen risks, (47), and glyphosate disrupts that function. (46) 

So the spike protein may be disruptive to microtubules - or it may be that some people have microtubules made with glyphosate that are more prone to malfunction when they are needed for the immune functions, or it may be a combination of both - spike protein disrupts microtubules, particularly those that contain glyphosate.

Microtubules are the scaffolding and cranes of our extracellular and intracellular matrix - which is gelatin like - watery and fluid but with some solidity too. Microtubules direct activity by connecting things where they need to be. If the hook end of the crane is dysfunctional - then nothing can be moved around, such as a pathogen needing to be sent to a white blood cell for removal perhaps. - overview, not my specialty except they are essential for cellular division and growth of all cells.

Histamine excess and mast cell over-activity can add to membrane breakdown and cause other odd symptoms unrelated to skin health.

Histamine excess and mast cell over-activity would be caused by an allergy like response to the spike protein. 

Histamine excess can also be due to an increase in activation of vitamin A and carotenoids to retinoic acid which can occur as a response to a viral infection or immune challenge (a vaccine). Liver enzymes activate any vitamin A or carotenoids into the active retinoic acid form, with no modulation for need. Retinoic acid activates allergy response mast cells, a type of immune cell that tends to line membrane areas of the body - skin reactions or GI upset. 

Histamine output occurs from the activated mast cell. Excess histamine causes allergy symptoms that are common to seasonal allergies, runny nose, itchy eyes. When more severe it can also cause mood or behavior changes that would be mistaken as psychiatric problems rather than an allergy response in overdrive. The solution is avoid vitamin A and carotenoid rich food sources - for life. Over time the excess histamine may lead to kidney or liver damage and schizophrenia or Alzheimer's possibly. 

Retinoid toxicity may be a long term problem following the change in the liver to enzymes that activate vitamin A. The problem has been seen after EBV viral infections with Chronic Fatigue type symptoms. Skin rashes can also occur and many odd symptoms. 

Histamine is a modulator during normal health, helping to balance an overactive or underactive brain response. Excess histamine leads to an overactive escalation of thoughts and can cause loss of rational control. Timeout to quiet the brain is needed. Pomegranate products can help as the phytonutrients inhibit mast cells. Apple, fennel, celery, quercetin, luteolin, are other foods and nutrients that may have a calming effect during histamine excess.

• See: Glyphosate increases histamine, both may be a factor in COVID19
• For an overview and links to further information about Mast Cell overactivity and histamine excess see: MCAS/Histamine. 
• Histamine Food List & other diet related inflammatory issues - Alkaline/acid producing foods, oxalates, lectins.
• Retinoid Toxicity.

Glyphosate as a risk factor for more severe COVID-19 has been theorized by Stephanie Seneff to involve the enzyme heme-oxygenase-1. In normal function it is anti-inflammatory and production of it is stimulated by anti-inflammatory Nrf2 (a protein & its gene). If the protein chain for heme-oxygenase-1 is made with glyphosate instead of glycine then potentially the new folded protein it forms might be defective - or it may function in a different way. 

Stephanie Seneff suggests that the enzyme, when made with glyphosate, may be acting as an inflammatory peroxidase instead of functioning as a beneficial anti-inflammatory enzyme. And there would be a positive feedback loop - more inflammation -> more call to make more heme-oxygenase-1, normally an anti-inflammatory, except the malfunctioning (glyphosate containing) peroxidase may be made instead, increasing inflammation more -> Nrf2 call for more production of heme-oxygenase-1 -> more glyphosate peroxidase is made -> more inflammation, etc.

The garlic, and sulforaphane containing produce can act as Nrf2 promoters which might also lead to increased heme-oxygenase-1, since it is part of the anti-inflammatory pathways, and which in normal function acts as an anti-inflammatory enzyme. The citrus and pomegranate polyphenols might also increase Nrf2 and heme-oxygenase-1 production. >>> test urine for glyphosate seems like an essential first step before proceeding with other standard anti-inflammatory treatments other than vitamin C and quercetin – both of which can help reduce an overexpression of Nrf2 (which occurs in a few types of cancer). (30) 

• See the post: Glyphosate and COVID-19.

Disclaimer: This information is provided for educational purposes within the guidelines of fair use. While I am a Registered Dietitian this information is not intended to provide individual health guidance. Please see a [*functional] health professional for individual health care purposes.